Checkpoint Kinase 2 (CHEK2) Peptide
CHEK2
Reactivité: Humain
Hôte: Synthetic
BP, WB, IHC
N° du produit ABIN974905
Aperçu rapide pour Checkpoint Kinase 2 (CHEK2) Peptide (ABIN974905)
Antigène
CHEK2
(Checkpoint Kinase 2 (CHEK2))
Origine
Humain
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB), Immunohistochemistry (IHC)
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Attributs du produit
- This is a synthetic peptide designed for use in combination with anti-CHEK2 antibody (Catalog #: ARP30290_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Indications d'application
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
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Stock
- -20 °C
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Stockage commentaire
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- CHEK2 (Checkpoint Kinase 2 (CHEK2))
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Sujet
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In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: RP11-436C9.1, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1
Protein Interaction Partner: ATM,ATM,AATF,ASF1A,ATM,ATM,ATR,BRCA1,CDC25A,CDC25C,DBF4,E2F1,ERCC6,KPNA2,MDC1,MDM2,MDM4,MRC1,MSH2,MUS81,NBN,PLK1,PLK3,PPP2R2A,PPP2R2B,PPP2R5A,PPP2R5B,PPP2R5C,PPP2R5D,PPP2R5E,RAD9A,TP53,TSSK1B,ATMIN,BRCA1,BRCA2,CDC25A,GINS2,MDC1,MDM2,MSH2,MUS81,PLK1,PLK3,RAD50,TP53BP1,VCP
Protein Size: 514 -
Poids moléculaire
- 58 kDa
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ID gène
- 11200
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NCBI Accession
- NM_145862, NP_665861
Antigène
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